Table 1. Table describing the HPLC standard operating procedure for Cy3-PNA-hsiRNA hybrid concentration estimation.

Time	Buffer concentration	Step
0 min	90% A, 10% B
2 min	90% A, 10% B	Equilibration, free PNA should come around 2 min (void volume)
10 min	50% A, 50% B	Elution, PNA-hsiRNA hybrid should come around 40% B, 7.5-8.5 min
10.5 min	100% B
12.5 min	100%B	Wash
13 min	90% A, 10% B
15 min	90% A, 10% B	Regeneration