Fig 3. Utilization of scoring system to quantitate disease progression and discriminate between severity of eyelid disease induced by level of total body irradiation (TBI) conditioning.

Animals were conditioned with low (7.5Gy) or high (10.5 Gy) TBI and transplanted as in Fig. 2. The scoring system (Table 1) was applied to monitor and score the development of oGVHD. A) Clinical lid margin score with 10.5Gy BM+T cells demonstrated significant eyelid involvement relative to control, with peak score at week 7. In contrast, mice with low conditioning exhibited marginal changes vs. control, B) eGFP lid margin score in mice receiving high conditioning exhibited significantly more eGFP cell infiltrate compared to control. 105Gy+ T cells reached near peak infiltrate by week 4. Similarly to clinical scoring, mice receiving low conditioning exhibited marginal changes. C) Quantification of eGFP by MGI also demonstrated significant changes in high but not low conditioned animals. Mean values are mean score +/− SEM of data from all mice in the injected T cell or control groups. *p < .05. **p < .0.01, paired t-test