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. 2017 May 24;8(32):52016–52025. doi: 10.18632/oncotarget.18152

Figure 2. T. brucei mediated apoptosis of 5T33MM cells in vivo.

Figure 2

7 days after inoculation with 5T33MM cells, mice were divided into 2 groups. One group was infected with T. brucei (n = 12, pooled to 4 samples), the other served as control (n = 3). Mice were sacrificed at day 20. 5T33MM cells were isolated from the bone marrow and purified to > 85% by depletion of CD11b+ cells with MACS sorting. Western blot analysis for pJNK, JNK, MCL-1, Bim EL/L/S, Bax, caspase-8, caspase-9, caspase-3 and PARP was performed. β-tubulin served as loading control.