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. 2017 May 24;8(32):52016–52025. doi: 10.18632/oncotarget.18152

Figure 3. Effects of T. brucei on unfolded protein response pathways in 5T33MM cells.

Figure 3

A.-B. 7 days after inoculation with 5T33MM cells, mice were divided into 2 groups. One group was infected with T. brucei (n = 12, pooled to 4 samples), the other served as control (n = 3). Mice were sacrificed at day 20. 5T33MM cells were isolated from the bone marrow and purified to > 85% by depletion of CD11b+ cells with MACS sorting. Western blot analysis for PERK, ATF4, CHOP, IRE1, GRP-78, caspase-12, pEIF2A, EIF2A, XBP-1 was performed. β-tubulin served as loading control. B. From 2 samples of each group as depicted in A, further analysis was performed for ubiquinated proteins. β-tubulin served as loading control. C. Viability assay was performed for 24h with MM cells (purity > 90%) of 3 different mice. Cells were treated with a combination of a PERK and IRE1α inhibitor. *means p < 0.05. D. One experiment representing 3 is shown of MM cells (purity > 90%) which were treated with the combination of PERK and IRE1α inhibitors for 21 hours, followed by western blot analysis.