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. 2016 Jul 16;8(32):52104–52117. doi: 10.18632/oncotarget.10631

Figure 6. Transfection of miR-375 mimics reduces HPV oncogene levels in CaSki and SiHa cells.

Figure 6

(A) Expression of endogenous E6*I and E7 mRNA in CaSki and SiHa cells transfected with miR-375 mimics was quantified using RT-qPCR. Final concentration of 100 nM was used for miR-375 mimics as well as 25 nM for non-specific control miRNAs. The results are indicated as mean E6*I and E7 mRNA levels measured 48 hours after transfection and the according standard deviation is represented by the error bars. P values were calculated by utilizing Student`s t-test using non-specific control as a reference. *p < 0.05, **p < 0.01 and ns: not significant. (B) E7 protein levels in CaSki and SiHa cells 48 hours after transfection with miR-375. Final concentration of 100 nM was used for miR-375 mimics as well as 25 nM for non-specific control miRNAs. Actin was used as an internal control.