Table 2. Primer sequences used for methylation-specific and RT qPCR as well as for pyrosequencing.

Name	Forward (5′ - 3′)	Reverse (5′ - 3′)	Amplicon (bp)
Primers for RT-qPCR
HPV 16 E6*I	ACT GCG ACG TGA GGT GTA TTA AC	TGG AAT CTT TGC TTT TTG TCC	85
HPV 16 E7	CAG CTC AGA GGA GGA GGA TG	GCC CAT TAA CAG GTC TTC CA	166
HPV 18 E6*I	TGT ATA TTG CAA GAC AGT ATT	GCT GGA TTC AAC GGT TTC TGG	249
HPV 18 E7	CCC CAA AAT GAA ATT CCG GT	GTC GCT TAA TTG CTC GTG ACA TA	51
β-actin	ATG TGG CCG AGG ACT TTG ATT	AGT GGG GTG GCT TTT AGG ATG	107
Primers for methylation-specific qPCR
Hsa-miR-375 gene	GGG GCG TTG TGT AGT ATT GAG TTC	GAA ACG AAA ACG AAA AAC CCG	91
β-actin gene	TGG TGA TGG AGG AGG TTT AGT AAG T	AAC CAA TAA AAC CTA CTC CTC CCT TAA	133
Primers for bisulfite-based pyrosequencing of HPV 16 E2BS 3 and 4
Amplification primers	TTG TAA AAT TGT ATA TGG GTG TG	Bio- AAA TCC TAA AAC ATT ACA ATT CTC	180
Sequencing primer	AAT TTA TGT ATA AAA TTA AGG G