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. 2016 Jun 17;8(32):52118–52131. doi: 10.18632/oncotarget.10130

Figure 3. Quercetin inhibits Cr(VI)-induced miR-21 elevation and PDCD4 reduction.

Figure 3

BEAS-2B cells were treated with Cr(VI) (5 μM) for 24 h in the presence of quercetin (0, 5, 10 μM). A. The relative miR-21 level was determined by Taqman real-time PCR. B. PDCD4 protein levels after acute Cr(VI) treatment was detected by immunoblotting C. Representative images of fluorescence immunostaining of PDCD4 D. Quercetin ameliorates the Cr(VI)-induced inhibition of PDCD4 3′-UTR reporter activity. BEAS-2B cells were transfected with renilla reporter construct (pGL3-PDCD4_3′-UTR), miR-21 inhibitor (100 nM), negative control (100 nM), and pGL3-promoters and treated with 5 μM Cr(VI) for 6 h in the presence of quercetin (10 μM). Cellular lysates were subjected to a luciferase reporter assay as described in Materials and Methods. The results are expressed as relative activity (relative luminescence units (RLU)) normalized to the luciferase activity in the vector control cells without treatment. Data presented in the bar graphs are the mean ± SD of three independent experiments. *indicates a statistically significant difference from control cells with p<0.05.