Figure 6. SF1126 inhibits tumor growth of MYCN amplified CHLA-136 xenografts in vivo.
(A) 5 × 106 CHLA-136-Fluc cells implanted subcutaneously in NSG mice. After 15 days of tumor implantation, mice were imaged on Xenogen IVIS-200 system & mice were randomized into vehicle & SF1126 treated groups. Mice were treated with SF1126 (50 mg/kg/day five days a week) for 3 weeks, until tumors were harvested. Figure shows the longitudinal images of a representative mouse from each group visualized on day 0 and day 21 (of SF1126 treatment started) using bioluminescence imaging. (B) Quantitative analysis of bioluminescence signals in Vehicle and SF1126 treated group using IVIS Spectrum. (C) Western blot analysis of pAKT, MYCN and Cyclin D1 on tumors isolated from the SF1126- or Vehicle-treated mice in A.