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. 2017 Apr 10;8(32):52345–52356. doi: 10.18632/oncotarget.17017

Figure 2. Cisplatin increases ALDH3A1 expression in HNSCC.

Figure 2

(A) Western blot analyses of human primary tumor homogenates from HNSCC patients using GAPDH as a loading control. (B) SCC4 and PCI-13 cells were treated with cisplatin (15 μM) for 2 and 4 days and total cell lysates were analyzed by Western blot for ALDH3A1 protein. Densitometric analysis of ALDH bands obtained by Western blot using Image J software shows relative levels after normalization for equal protein loading using β-tubulin as a loading control. Results are expressed as mean±SEM. (*p < 0.05 vs. respective control, t-test).