Table 5. Primer sequences for quantitative real-time polymerase chain reaction.
Gene | Sequence |
---|---|
miR-126 | F: 5′- ACACTCCAGCTGGGTCGTACCGTGAGTAAT-3′ |
R: 5′- TGGTGTCGTGGAGTCG-3′ | |
EGFL7 | F: 5′-CAACCCGACAGGAGTGGACAGT-3′ |
R: 5′-TCACGAGTCTTTCTTGCAGGAGC-3′ | |
ERK | F: 5′-GGCACCAACCATTGAGCAGA-3′ |
R: 5′-GATCATTGCTGAGGTGCTGTGTC-3′ | |
Fas | F: 5′-GCATCTGGACCCTCCTACCTCTG-3′ |
R: 5′-GACAAAGCCACCCCAAGTTAGA-3′ | |
FasL | F: 5′-TTCTGGTTGCCTTGGTAGGATTG-3′ |
R: 5′-ACCTTGAGTTGGACTTGCCTGTT-3 | |
Caspase-3 | F: 5′-TACCACGCCACCACCGGCCCA-3′ |
R: 5′- GGCATTTTGGCTGTCGTCAGGGAA-3′ | |
Bcl-2 | F: 5′-ATGTGTGTGGAGAGCGTCAA-3′ |
R: 5′-ACAGTTCCACAAAGGCATCC-3′ | |
GAPDH | F: 5′-AGAAGGCTGGGGCTCATTTG-3′ |
R: 5′-AGGGGCCATCCACAGTCTTC-3′ |
Note: miR-126, microRNA-126; F, forward; R, reverse.