Figure 2. Effects of chemoresistance on the drug transporters and EMT.

(A) IC₅₀ values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E. Data from three independent experiments were graphed as the mean ± SD. *P < 0.05.