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. 2017 May 2;8(32):52913–52922. doi: 10.18632/oncotarget.17577

Figure 3. Zbtb7a is the direct target of miR-106b.

Figure 3

(A and B) Luciferase activity of the reporter construct containing wild type or mutant 3′UTR of Zbtb7a was measured after co-transfection with 100 nM miR-106b mimics (A) or anti-miR-106b mimics (B). (C) The Zbtb7a mRNA level was measured in M139 cells after transfection with 100 nM miR-106b mimics or anti-miR-106b mimics. (D) The Zbtb7a protein level was measured by western blot assay in M139 cells after transfected with miR-106b mimics and anti-miR-106b mimics. (E) The mRNA expression level of Zbtb7a in primary CCA tumors and recurrent CCA tumors was examined by q-PCR. (F) The correlation of miR-106b and Zbtb7a in CCA recurrent tumors was measured by q-PCR. All data represent the means ± SEM of three independent experiments. * represents p<0.05, ** represents p<0.01, *** represents p<0.001.