Figure 4. Zbtb7a is the functional target of miR-106b in modulating CCA cells sensitive to 5-FU.

(A and C) The growth of M139 (A) and M214 (C) cells, which were stably expressing miR-106b and Zbtb7a, were measured by MTT assays. (B and D) The apoptotic M139 (B) and M214 (D) cells were examined by flow cytometry after these cells were transduced with miR-106b and Zbtb7a and then treated with 40 μM 5-FU. (E and F) Zbtb7a rescues colony formation of MMU-139 and MMU-214 cells. (G and I) Stably miR-106b and Zbtb7a expressing M139 (G) and M214 cells (I) were inoculated into BALB/c nude mice. Tumor volume was measured every 5 days when the tumor reaching 100 mm³. (H and J) Tumor weight was measured at the end point of these experiments. All data represent the means ± SEM of three independent experiments. * represents p<0.05, ** represents p<0.01, *** represents p<0.001.