Table 2. Sequences of the qRT-PCR primers.
Gene | Sequence |
---|---|
β-catenin | Upstream: 5′-GCTACTCAAGCTGATTTGATGGA-3′ Downstream: 5′-GGTAGTGGCACCAGAATGGATT-3′ |
GSK-3β | Upstream: 5′-AACACCAACAAGGGAGCAAA-3′ Downstream: 5′-GAGCGTGAGGAGGGATAAGG-3′ |
LC3 | Upstream: 5′-GAGAAGCAGCTTCCTGTTCTGG-3′ Downstream: 5′-GTGTCCGTTCACCAACAGGAAG-3′ |
Beclin1 | Upstream: 5′-AACCAGATGCGTTATGCCC-3′ Downstream: 5′-ATTGATTGTGCCAAACTGTCC-3′ |
GAPDH | Upstream: 5′-GGATTTGGTCGTATTGGG-3′ Downstream: 5′-GGAAGATGGTGATGGGATT-3′ |
qRT-PCR: quantitative real-time PCR; GSK-3β: glycogen synthase kinase-3β; LC3: light chain 3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.