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. 2017 May 8;8(32):52960–52974. doi: 10.18632/oncotarget.17674

Table 2. Sequences of the qRT-PCR primers.

Gene Sequence
β-catenin Upstream: 5′-GCTACTCAAGCTGATTTGATGGA-3′
Downstream: 5′-GGTAGTGGCACCAGAATGGATT-3′
GSK-3β Upstream: 5′-AACACCAACAAGGGAGCAAA-3′
Downstream: 5′-GAGCGTGAGGAGGGATAAGG-3′
LC3 Upstream: 5′-GAGAAGCAGCTTCCTGTTCTGG-3′
Downstream: 5′-GTGTCCGTTCACCAACAGGAAG-3′
Beclin1 Upstream: 5′-AACCAGATGCGTTATGCCC-3′
Downstream: 5′-ATTGATTGTGCCAAACTGTCC-3′
GAPDH Upstream: 5′-GGATTTGGTCGTATTGGG-3′
Downstream: 5′-GGAAGATGGTGATGGGATT-3′

qRT-PCR: quantitative real-time PCR; GSK-3β: glycogen synthase kinase-3β; LC3: light chain 3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.