Figure 4. Combination of miR-133b and 5-FU or VCR induced multidrug resistant cell lines necrosis.

The flow cytometric result is divided into four quadrants by lines. The upper left quadrant (Annexin V-FITC⁻/PI⁺ staining) is necrotic cells; the upper right quadrant (Annexin V-FITC⁺/PI⁺ staining) a late apoptotic cell; the left lower quadrant (Annexin V-FITC⁻/PI⁻ staining) is the normal activity of cells; the lower right quadrant (Annexin V-FITC⁺/PI⁻ staining) are early apoptotic cells. Thus, the rate of necrotic cells significantly increased in miR-133b group. The data are presented as the mean ± standard deviation. The results are representative of three independent experiments. *P<0.05.