Expression of the rm-hsc71m gene
is modulated during early development. (A) Expression
of rm-hsc71m during early development. Total RNAs
were prepared from developing embryos or larvae at each stage of
development (n = 3–10) and used
to monitor the level of rm-hsc71m expression by
RT–PCR using a set of rm-hsc71m-specific
primers in exon II and exon IV, respectively (rm-hsc71m primer c/d
in Table 1). Staging of embryos was determined by the criteria of Harrington
(34). As a loading control, photographs of ethidium bromide-stained
28S and 18S rRNA are also shown in the lower panel. Lane 1, unfertilized
eggs; lane 2, blastula embryos; lane 3, 2-day-old embryos; lanes
4–12, 6- to 14-day-old embryos; lane 13, hatched larvae;
lane 14, juvenile stage larvae. (B) Lateral views of
a whole-body of a 3-day-old (a) and a 13-day-old embryo (b) showing
spatial expression patterns of rm-hsc71m detected
by whole-mount in situ hybridization with an antisense
RNA probe containing a 414 base sequences corresponding to exons
II–IV of rm-hsc71m (see Materials and
Methods). Control 13-day-old embryos (c) were hybridized with a
sense probe. High expression of rm-hsc71m in the
head-fold and trunk region is marked by an arrowhead. Bar, 100 µm.