Figure 2.

Expression of the rm-hsc71m gene is modulated during early development. (A) Expression of rm-hsc71m during early development. Total RNAs were prepared from developing embryos or larvae at each stage of development (n = 3–10) and used to monitor the level of rm-hsc71m expression by RT–PCR using a set of rm-hsc71m-specific primers in exon II and exon IV, respectively (rm-hsc71m primer c/d in Table 1). Staging of embryos was determined by the criteria of Harrington (34). As a loading control, photographs of ethidium bromide-stained 28S and 18S rRNA are also shown in the lower panel. Lane 1, unfertilized eggs; lane 2, blastula embryos; lane 3, 2-day-old embryos; lanes 4–12, 6- to 14-day-old embryos; lane 13, hatched larvae; lane 14, juvenile stage larvae. (B) Lateral views of a whole-body of a 3-day-old (a) and a 13-day-old embryo (b) showing spatial expression patterns of rm-hsc71m detected by whole-mount in situ hybridization with an antisense RNA probe containing a 414 base sequences corresponding to exons II–IV of rm-hsc71m (see Materials and Methods). Control 13-day-old embryos (c) were hybridized with a sense probe. High expression of rm-hsc71m in the head-fold and trunk region is marked by an arrowhead. Bar, 100 µm.