rm-hsc71m expression
is differentially regulated in a tissue-specific manner. (A)
Expression profile of rm-hsc71m in adult tissues.
Total RNAs were isolated from several organs of a 1-year-old fish
and subjected to RT–PCR analysis using a set of rm-hsc71m-specific
primers as described in the legend to Figure 2A. The lower panel
shows photographs of ethidium bromide-stained 28S and 18S rRNAs
as a loading control. (B) Views of paraffin-sectioned
1-year-old fish showing spatial expression patterns of rm-hsc71m detected
by in situ hybridization with antisense RNA probes
as described in Figure 2B. Sections were stained with hematoxylin/eosin
(a, d, f and h) or hybridized with an antisense (b, e, g and i) rm-hsc71m RNA probe. As a negative hybridization
control, a sense rm-hsc71m RNA probe was also employed
(c). Arrows indicate regions showing strong expression of rm-hsc71m (open
arrows in panels e, g and i indicate skeletal muscle tissue showing
the strongest expression). B, brain; CL, cerebellum (corpus cerebelli);
E, esophagus; GF, gill filament; I, infundibulum; L, liver; MO,
medulla oblongata; O, oral cavity; OL, olfactory lobe (telencephalon);
ON, optic nerve; OT, optic tectum; S, spine; SK, skeletal muscle.
Scale bars: a–c, 1.25 µm; d–i,
0.32 µm.