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. 2017 Jun 21;8(32):53154–53167. doi: 10.18632/oncotarget.18587

Figure 2. Statins regulate expression of cell cycle genes.

Figure 2

(A-D) PANC1 cells treated with 10 μM of each of 9 statins for 48 hours were harvested for RT-qPCR for CCNA2, TOP2A, AURKA, and p21, shown as fold change over DMSO treated cells. (E-I) To extend the studies to additional cell lines, PANC1, BxPC3 and a low passage line derived from a patient's tumor, 779e, were treated with 10uM pitavastatin for 48 hours. RT-qPCR analysis of (E) CCNA2, (F) TOP2A and (G) AURKA revealed significantly reduced mRNA expression in all three lines. Conversely the tumor suppressor p21 was significantly induced by pitavastatin at the (H) mRNA level, and (I) the protein level in all three lines. Data are represented as mean +/−SD, *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001.