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. 2017 May 29;8(32):53180–53193. doi: 10.18632/oncotarget.18261

Figure 3. Direct regulation of ANLN by miR-217 in PDAC cell lines.

Figure 3

(A) ANLN mRNA expression in PDAC cell lines was evaluated by qRT-PCR 72 h after transfection with miR-217. GUSB was used as an internal control. *, P < 0.0001. (B) ANLN protein expression in PDAC cell lines was evaluated by Western blot analyses 96 h after transfection with miR-217. GAPDH was used as a loading control. (C) miR-217 binding sites in the 3′-UTR of ANLN mRNA. Dual luciferase reporter assays using vectors encoding putative miR-217 (positions 132 - 139 or 660 - 666) target sites of the ANLN 3′-UTR for both wild-type and deleted regions. Normalized data were calculated as ratios of Renilla/firefly luciferase activities. *, P < 0.005.