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. 2017 Jun 21;8(32):53518–53530. doi: 10.18632/oncotarget.18591

Figure 4. SHP2 depletion upregulated the expression of HLA-ABC and PD-L1 in PC3 and DU145 cells.

Figure 4

PC3 and DU145 cells were transfected with SHP2 siRNA (50 nmol/mL) for 48 hours. IFN-γ was used as a positive control to induce the expression of HLA-ABC and PD-L1. (A) A representative flow histogram of HLA-ABC is shown. (B) HLA-ABC MFI fold change of PC3 and DU145 after treatment with SHP2 siRNA or IFN-γ for 48 hours. Bar graph shows the MFI results represented as mean ±SE. (C) A representative flow histogram of PD-L1 of PC3 cells is shown. (D) PD-L1 MFI fold change of PC3 and DU145 after transfection of SHP2 siRNA or treatment with IFN-γ for 48 hours. Bar graphs represent mean ±SD from duplicate samples in three independent experiments. Full black line depicts isotype group. Full gray line depicts control siRNA group. Black line depicts SHP2 siRNA group. Dashed line depicts IFN- γ group.