Vehicle-treated aS-expressing flies (w; +; UAS-Hsap/nSyb-Gal4; AS VEH, white bars) and control flies (w+; +; +/nSyb-GAL4; CTRL VEH, gray bars). (A) Mean velocity (mm/s). (B) Maximum velocity (mm/s). (C) Total walking duration (s). (D) Total walking distance (mm). (E) Percentage of time that flies are in motion (%). (F) Mean trajectory length (mm). (G) Number of fly walking trajectories. (H) Mean trajectory length per episode (mm). Bars represent mean values (n = 10). Error bars indicate ± SE. * P < 0.05; ** P < 0.01; *** P <0.001. Tested compounds were either FN075 (AS FN075, magenta bars), MS400 (AS MS400, green bars) or C10 (AS C10, blue bars) at 100μM concentration.