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. 2017 Aug 22;13(8):e1006955. doi: 10.1371/journal.pgen.1006955

Fig 6. YUC8/REIN7 is mainly required for IPA-dependent auxin biosynthesis.

Fig 6

(A) The simplified liner pathway of IAA biosynthesis. The red arrows indicate the function of YUC. Enzymatic assays with GST-AtYUC2, GST- REIN7, GST-REIN7m and the product IAA (B), the substrate TAM (C) were analyzed by LC-ESI-MS/MS. The bars represent ± SD from three independent experiments. (D) The content of remained NADPH in (C). The bars represent ± SD from three independent experiments. (E) Expression of auxin-inducible genes in 7-d-old normal grown seedlings. The bars represent ± SD from five independent experiments. (F) IAA content of 7-d-old normal grown seedlings. The bars represent ± SD from three independent experiments. ** indicates a significant difference compared to KT or HY at P < 0.01.