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. 2017 Aug 22;13(8):e1006955. doi: 10.1371/journal.pgen.1006955

Fig 8. Mutation of OsEIL1 abolishes ethylene-induced YUC8/REIN7 transcription.

Fig 8

(A) Expression of YUC8/REIN7 in response to ethylene. The wild-type seedlings were grown in the dark for 3 d and then treated with 10 ppm ethylene. The RNAs from roots and shoots were isolated and used for qPCR. (B) Ethylene-induced GUS activity in roots of transgenic plants harboring REIN7p-GUS. Etiolated seedlings of 3-d-old plants were treated with or without 10 ppm ethylene for 8 h before GUS activity was assayed. Bar = 10mm. (C) Root tip and elongation zone in (B). ‘RT’ represents root tip, ‘EZ’ represents elongation zone. Bar = 100 μm. (D) qPCR analysis of YUC8/REIN7 expression in primary roots and shoots of Nip, oseil1 and EIL1-OX seedlings grown in the dark for 3 d. (E) Expression of YUC8/REIN7 in primary roots of Nip, oseil1 and EIL1-OX seedlings grown in the dark for 3 d and then treated with 10 ppm ethylene. The data are shown as the mean ± SD of five biological replicates. * and ** indicate significant differences compared to 0 h at P < 0.05 and P < 0.01, respectively.