Western analysis of the translation
products of wild-type C/EBPβ expression
vector (pCMV-C/EBPβ-wt), expression
vectors with mutations at multiple AUG start sites, and mutation
of the sORF-AUG. (A) Maps of the expression vectors
used in (B). The open box denotes the sORF; (B)
western blot analysis of proteins from COS-1 cells: lane 1, proteins
from control, non-transfected COS-1 cells; lane 2, proteins from
cells transfected with control vector (pCMV); lanes 3–12,
proteins from cells transfected with the vector indicated. (C)
Comparison of the pool levels of C/EBPβ isoforms
produced by each expression vector. Protein loading was adjusted
to reflect variations in vector mRNA pool levels, and translation
from an equivalent mRNA pool level. Data for three independent transfections
are shown as mean ± SD. The numbering
of the grouped bars corresponds to the lanes in (B).