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. 2001 Jul 15;29(14):3087–3098. doi: 10.1093/nar/29.14.3087

Figure 4.

Figure 4

Western analysis of nuclear extracts from COS-1 cells transfected with wild-type C/EBPβ expression vector (pCMV-C/EBPβ-wt); expression vectors with mutations of the sORF-AUG and in which a 112-nt spacer was inserted between the termination site of the sORF and AUG-2. (A) Maps of the expression vectors used in these experiments. Vector mtsORF has a mutation changing the sORF-AUG to -UUG, sORF(K2) has a mutation changing the sORF to a perfect Kozak sequence, sORF(G8) is altered to produce a sORF peptide of one methionine and eight glycines rather than the normal MPPAAARRL, and spacer 112 has a 112-nt spacer inserted between the termination site of the sORF and AUG-2. (B) Western blot analysis of proteins from COS-1 cells: lane 1, proteins from non-transfected cells; lane 2, proteins from cells transfected with pMCV control vector; lanes 3–6, proteins from cells transfected with the expression vector indicated. (C) Summary of protein pool levels of C/EBPβ isoforms from several independent experiments as shown in (B), mean ± SD. The numbering of the grouped bars corresponds to the lanes in (B). (D) Western blot analysis of proteins from COS-1 cells, lanes labeled as in (B). (E) Summary of protein pool levels of C/EBPβ isoforms from several independent experiments as shown in (D), mean ± SD. The numbering of the grouped bars corresponds to the lanes in (D).