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. 2001 Jul 15;29(14):3087–3098. doi: 10.1093/nar/29.14.3087

Figure 5.

Figure 5

Functional analysis of the C/EBPβ sORF in alternate translational initiation. (A) Maps of the expression vectors used. The NewAUG vectors have a new in-frame AUG initiation codon inserted either alone, or in combination with mutations in the sORF start site (NewAUG-mtsORF), AUG-1 (NewAUG-mtAUG1) or AUG-2 (NewAUG-mtAUG2). Also shown are the maps for a series of chimeric constructs in which the C/EBPδ (in front of C/EBPδ AUG-1). Both the wild-type (β-sORFwt-δ) and mutant (β-sORFmt-δ) sORF were used. (B) Western blot analysis of proteins from COS-1 cells: lane 1, proteins from non-transfected cells; lane 2, proteins from cells transfected with pMCV control vector; lanes 3–8, proteins from cells transfected with the expression vector indicated. (C) Western blot analysis of proteins from COS-1 cells: lane 1, proteins from non-transfected cells; lane 2, proteins from cells transfected with pMCV control vector; lanes 3–6, proteins from cells transfected with the expression vector indicated.