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. 2001 Jul 15;29(14):3087–3098. doi: 10.1093/nar/29.14.3087

Table 1. Expression vector mutations are listed under Constructs and the oligonucleotides used are listed under Oligonucleotides column.

Constructs
Oligonucleotides
Sequences
mtsORF AN1 5′-CCGCGTTCATGCACCGCCTGCTGGCCTGG-3′ (ATG-1 underlined)
  AN2 5′-cagcaggcggtgcatgaacgcggggcc-3′
  AN11 5′-GACGCAGCTTGCCTCCC-3′ (mutated ATG of sORF underlined)
  AN12 5′-CAAGCTGCGTCCCAGGC-3′
  AN5 5′-GCCGCCGCCCGCCGC-3′
  AN6 5′-GGCGGCGGCGGGAGG-3′
  AN7 5′-CTTTAGACC-3′ (stop codon of sORF underlined)
  AN8 5′-CATGGGTCTAAAGGCGGCG-3′
mtAUG-1 Beta-MT35/30-P1 5′-CCGCGGGCCCCGCGTTCTTGCACCGC-3′ (ATG-1 changed to TTG)
  Beta-P30-P 5′-GCTCGTAGTAGAAGTTGG-3′
mtAUG-2 Beta-WT-P 5′-CGGAGCCCGCGGGCCCCGCG-3′
  Beta-MT35/30-P2 5′-AAGTTGGCCACTTCCAAGGGTCTAAAG-3′ (ATG-2 changed to TTG)
AUG-1(K) Beta-ATG-1K 5′-CAGCGGAGCCCGCGGGCCCCGCGGCCATGGAC CGCCTGC-3′ (Kozak ATG-1 underlined)
  Beta-P30-P 5′-GCTCGTAGTAGAAGTTGG-3′
Spacer112 Beta-BN-P1 5′-GCTTGGATCCTCTAGAGTCCGAGC-3′ (BamHI site underlined)
  Beta-BN-P2 5′-TGATACTAGTCTAAAGGCGGCGCGCGGC-3′ (SpeI site underlined)
  Beta-Spacer112-P1 5′-GGCGGCTAGCCGAGAGACTTCTCGCGGAGCTG-3′ (NheI site underlined)
  Beta-Spacer112-P2 5′-TGAACCATGGGTTCGGTAGGAAAAGGAGCGAGT TTTG-3′ (NcoI site underlined)
sORF(K2) Beta-WT-P 5′-CGGAGCCCGCGGGCCCCGCG-3′
  ORF(K)-P1 5′-CATGGTGGCTCCCAGGCC-3′ (Kozak ATG of sORF underlined)
  ORF(K)-P2 5′-GGAGCCACCATGGCTCCCGCCG-3′ (Kozak ATG of sORF underlined)
  Beta-P30-P 5′-GCTCGTAGTAGAAGTTGG-3′
sORF(G8) Beta-WT-P 5′-CGGAGCCCGCGGGCCCCGCG-3′
  ORF(K)-P1 5′-CATGGTGGCTCCCAGGCC-3′ (Kozak ATG of sORF underlined)
  ORF(K)-G-F 5′-GGAGCCACCATGGGCGGCGGTGGCGGCGGTGG CGGCGACTACAAAG-3′ (Kozak ATG of sORF underlined)
  Beta-P30-P 5′-GCTCGTAGTAGAAGTTGG-3′
  ORF(K)-G 5′-AGTTGGCCACTTCCATGGGTCTAGCCGCCACCG CCGCCAC-3′
NewAUG Beta-WT-P 5′-CGGAGCCCGCGGGCCCCGCG-3′
  NewAUG1 5′-TCTCCGACGCCATGGCCGACGAC-3′
  NewAUG2 5′-GTCGTCGGCCATGGCGTCGGAGA-3′
  Csp-P 5′-GCTTGCAGTCCGCGGG-3′
δ-wt Delta-F1 5′-CGCCGACTGCCGGGACTACAAAGACGATGACG ATAAATAACG-3′ (Flag tag underlined)
  Delta-F2 5′-CGCGTTATTTATCGTCATCGTCTTTGTAGTCCCGGCAGTCGG-3′
(β-sORFwt)-C/EBPδ or (β-sORFmt)-C/EBPδ Delta-1 5′-ACGCCGCCATGGGCGCCGCGCTTTTCAGCCTG-3′ (NcoI site underlined)
  Delta-2 5′-CGGCGGCCATGGTGTCAATGTAGGCGCTGAAG-3′