Constructs
|
Oligonucleotides
|
Sequences
|
mtsORF |
AN1 |
5′-CCGCGTTCATGCACCGCCTGCTGGCCTGG-3′ (ATG-1
underlined) |
|
AN2 |
5′-cagcaggcggtgcatgaacgcggggcc-3′ |
|
AN11 |
5′-GACGCAGCTTGCCTCCC-3′ (mutated
ATG of sORF underlined) |
|
AN12 |
5′-CAAGCTGCGTCCCAGGC-3′ |
|
AN5 |
5′-GCCGCCGCCCGCCGC-3′ |
|
AN6 |
5′-GGCGGCGGCGGGAGG-3′ |
|
AN7 |
5′-CTTTAGACC-3′ (stop codon
of sORF underlined) |
|
AN8 |
5′-CATGGGTCTAAAGGCGGCG-3′ |
mtAUG-1 |
Beta-MT35/30-P1 |
5′-CCGCGGGCCCCGCGTTCTTGCACCGC-3′ (ATG-1
changed to TTG) |
|
Beta-P30-P |
5′-GCTCGTAGTAGAAGTTGG-3′ |
mtAUG-2 |
Beta-WT-P |
5′-CGGAGCCCGCGGGCCCCGCG-3′ |
|
Beta-MT35/30-P2 |
5′-AAGTTGGCCACTTCCAAGGGTCTAAAG-3′ (ATG-2
changed to TTG) |
AUG-1(K) |
Beta-ATG-1K |
5′-CAGCGGAGCCCGCGGGCCCCGCGGCCATGGAC CGCCTGC-3′ (Kozak
ATG-1 underlined) |
|
Beta-P30-P |
5′-GCTCGTAGTAGAAGTTGG-3′ |
Spacer112 |
Beta-BN-P1 |
5′-GCTTGGATCCTCTAGAGTCCGAGC-3′ (BamHI site underlined) |
|
Beta-BN-P2 |
5′-TGATACTAGTCTAAAGGCGGCGCGCGGC-3′ (SpeI site underlined) |
|
Beta-Spacer112-P1 |
5′-GGCGGCTAGCCGAGAGACTTCTCGCGGAGCTG-3′ (NheI site underlined) |
|
Beta-Spacer112-P2 |
5′-TGAACCATGGGTTCGGTAGGAAAAGGAGCGAGT TTTG-3′ (NcoI site underlined) |
sORF(K2) |
Beta-WT-P |
5′-CGGAGCCCGCGGGCCCCGCG-3′ |
|
ORF(K)-P1 |
5′-CATGGTGGCTCCCAGGCC-3′ (Kozak
ATG of sORF underlined) |
|
ORF(K)-P2 |
5′-GGAGCCACCATGGCTCCCGCCG-3′ (Kozak
ATG of sORF underlined) |
|
Beta-P30-P |
5′-GCTCGTAGTAGAAGTTGG-3′ |
sORF(G8) |
Beta-WT-P |
5′-CGGAGCCCGCGGGCCCCGCG-3′ |
|
ORF(K)-P1 |
5′-CATGGTGGCTCCCAGGCC-3′ (Kozak
ATG of sORF underlined) |
|
ORF(K)-G-F |
5′-GGAGCCACCATGGGCGGCGGTGGCGGCGGTGG CGGCGACTACAAAG-3′ (Kozak ATG of sORF underlined) |
|
Beta-P30-P |
5′-GCTCGTAGTAGAAGTTGG-3′ |
|
ORF(K)-G |
5′-AGTTGGCCACTTCCATGGGTCTAGCCGCCACCG
CCGCCAC-3′ |
NewAUG |
Beta-WT-P |
5′-CGGAGCCCGCGGGCCCCGCG-3′ |
|
NewAUG1 |
5′-TCTCCGACGCCATGGCCGACGAC-3′ |
|
NewAUG2 |
5′-GTCGTCGGCCATGGCGTCGGAGA-3′ |
|
Csp-P |
5′-GCTTGCAGTCCGCGGG-3′ |
δ-wt |
Delta-F1 |
5′-CGCCGACTGCCGGGACTACAAAGACGATGACG ATAAATAACG-3′ (Flag
tag underlined) |
|
Delta-F2 |
5′-CGCGTTATTTATCGTCATCGTCTTTGTAGTCCCGGCAGTCGG-3′ |
(β-sORFwt)-C/EBPδ or (β-sORFmt)-C/EBPδ |
Delta-1 |
5′-ACGCCGCCATGGGCGCCGCGCTTTTCAGCCTG-3′ (NcoI site underlined) |
|
Delta-2 |
5′-CGGCGGCCATGGTGTCAATGTAGGCGCTGAAG-3′ |