Fig. 1.

Timeline of experimental design. Above the line are procedures given to all mice (N = 560), which include maternal diet manipulation (SD = standard diet, WD = Western diet), weaning at 3 weeks and 6 days of wheel access in adulthood. Below the line are procedures for 100 “focal” families and one male offspring from each of those families, which includes twice-daily behavior checks during the first 3 weeks, monitoring of home-cage activity and food consumption, behavioral testing on an elevated plus maze, and maximal aerobic capacity (VO₂max). Each tick mark represents one week and asterisks indicate when mice were weighed (in black) and measured for fat and lean composition (in grey).