Prior inhibition of ADH, CYP2E1 and catalase by 4-MP, 3-AT or their combination (30 min) ameliorates ethanol-evoked myocardial dysfunction in conscious female rats. The line graphs show the time course of the changes in the maximum rate of rise of left ventricular pressure (dP/dtmax) caused by ethanol in the absence or presence of 4-MP (A), 3-AT (C) or their combination (E). The bar graphs (B, D and F) represent the areas under the curves in these groups, respectively. Values are mean ± SEM. Data were analyzed by one-way ANOVA followed by post-hoc Tukey’s t-test comparison. *p < 0.05, versus saline + saline. #p < 0.05, versus saline-ethanol.