Prior inhibition of ADH, CYP2E1 and catalase by 4-MP, 3-AT or their combination (30 min) ameliorates ethanol-evoked myocardial dysfunction in conscious female rats. The line graphs show the time course of the changes in left ventricular developed pressure (LVDP) caused by ethanol in the absence or presence of 4-MP (A), 3-AT (C) or their combination (E). The bar graphs (B, D and F) represent the areas under curves in these groups, respectively. Values are mean ± SEM. Data were analyzed by one-way ANOVA followed by post-hoc Tukey’s t-test comparison. *p < 0.05, versus saline-saline.