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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Adv Mater. 2017 Jun 28;29(33):10.1002/adma.201701644. doi: 10.1002/adma.201701644

Figure 2.

Figure 2

Synthesis and characterization of hemolytic secreted protein (hSP)-loaded nanotoxoid, denoted nanotoxoid(hSP). A) Hemolysis of RBCs when incubated with varying amounts of hSP in the absence or presence of 400 μg of RBC nanosponges (n = 3; mean ± SD). B) Size of RBC nanosponges and nanotoxoid(hSP) as measured by dynamic light scattering (n = 3; mean ± SD). C) Surface zeta potential of nanosponges and nanotoxoid(hSP) (n = 3; mean ± SD). D) Transmission electron microscope image of nanotoxoid(hSP) negatively stained with uranyl acetate (scale bar = 100 nm). E) Dot blots probing for α-toxin, PVL, or γ-toxin in hSP, blank nanosponges, nanotoxoid(hSP), or nanotoxoid(hSP) subject to a wash step. F) Relative band intensities of western blots probing for α-toxin, PVL, or γ-toxin in nanotoxoid(hSP) or nanotoxoid(hSP) subject to a wash step (n = 3; mean ± SD). G) Retention of α-toxin, PVL, or γ-toxin on nanotoxoid(hSP) after dialyzing against 1× PBS for 48 hours (n = 3; mean ± SD).