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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Vasc Surg. 2017 Mar 1;67(3):910–921. doi: 10.1016/j.jvs.2017.01.021

Figure 5. Effects of chloroquine (CQ) on cultured C2C12 myoblasts.

Figure 5

A) ELISA was used to determine HMGB1 concentration in the supernatant of CQ treated C2C12 myoblasts. *P<.05, **p<.005 compared to no CQ; N=3 experiments performed in triplicate, t-test. B) Western blot of C2C12 myoblast lysates (B) and supernatants (C) after exposure to CQ at designated concentration for 18 hours. Lysates were assessed for P62/SQSTM1, LC3I-II conversion, HMGB1 and actin expression. Supernatants were concentrated and evaluated for HMGB1 expression. D) WB showing procaspase-1 and activated caspase-1 (20Kd subunit) from C2C12 myoblasts treated with CQ at designated concentration. E) LC3II and F) activated caspase-1 expression normalized to β-actin in response to CQ is shown relative to untreated cells. *P<.05; N=3 experiments performed in triplicate, t-test.