Figure 5.

Titrations of compounds and the corresponding IC₅₀. (A) Titration of Orlistat, a pancreatic lipase inhibitor that was identified in the primary screen. Increasing amounts of Orlistat was included during growth of 17978/plipBA. After overnight incubation in 96-well plates, the substrate 4-nitrophenyl myristate was added and the lipase activity was measured. Activities have been normalized to OD₆₀₀ of the cultures. n = 3, bars represent standard deviation from the mean. In follow-up counter screening, Orlistat was classified as an inhibitor of LipA activity (B) Titration of Oxyclozanide, an anthelmintic that was identified in the primary screen. Lipase activity in the presence of Oxyclozanide was measured as above. Activities have been normalized to OD₆₀₀ of culture. n = 3, bars represent standard deviation from the mean. Following counter screening, Oxyclozanide was classified as an inhibitor of lipA expression from the plasmid.