Table 2.

Optimization of assay conditions for development of a HTS.

Variables	Conditions tested	Final assay condition
Plate format	96 well vs. 384 well	384 well
Start OD	0.005, 0.0025, 0.001, 0.0005	0.005
Media	LB vs. Mueller-Hinton (MH)	MH
Volume	10, 15, 20, or 30 μL	10 μL
Temperature	20°, 24° (RT), 30°, 37°C	RT
Aeration	Shaking vs. non-shaking	Non-shaking
Substrate Concentration	0.225, 0.45, 0.9, 1.8 mM	0.45 mM