Table 1.
Primer sequences and PCR fragment size of tested MLS resistance genes.
| Gene | Primers sequence (5′–3′) | PCR fragment size (bp) | Reference |
|---|---|---|---|
| ermA | F: TCTAAAAAGCATGTAAAAGAA | 645 | (10) |
| R: CTTCGATAGTTTATTAATATTAG | |||
| ermB | F: GAAAAGTACTCAACCAAATA | 639 | (10) |
| R: AGTAACGGTACTTAAATTGTTTA | |||
| ermC | F: TCAAAACATAATATAGATAAA | 642 | (10) |
| R: GCTAATATTGTTTAAATCGTCAAT | |||
| msrA | F: GGCACAATAAGAGTGTTTAAAGG | 940 | (11) |
| R: AAGTTATATCATGAATAGATTGTCCTGTT | |||
| msrB | F: TATGATATCCATAATAATTATCCAATC | 595 | (11) |
| R: AAGTTATATCATGAATAGATTGTCCTGTT | |||
| lnuA | F: GGTGGCTGGGGGGTAGATGTATTAACTGG | 323 | (12) |
| R: GCTTCTTTTGAAATACATGGTATTTTTCGATC | |||
| lnuB | F: CCTACCTATTGTTTGTGGAA | 925 | (12) |
| R: ATAACGTTACTCTCCTATTC | |||
| lsaA | F: GGCAATCGCTTGTGTTTTAGCG | 1,200 | (13) |
| R: GTGAATCCCATGATGTTGATACC |
MLS, macrolides, lincosamides, and streptogramins; PCR, polymerase chain reaction.