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. 2017 Aug 28;8:1037. doi: 10.3389/fimmu.2017.01037

Figure 1.

Figure 1

Sirt2 is strongly expressed in myeloid cells. (A,B) Sirt1-7 mRNA levels in bone marrow (BM), BM-derived macrophages (BMDMs) and dendritic cells (BMDCs), and Flt3L-derived DCs (A) and RAW 264.7 macrophages (B) analyzed by RT-PCR and normalized to Hprt mRNA levels. Data are means ± SD of triplicate samples from one experiment performed with four BALB/c mice (A) or three preparations of RAW 264.7 macrophages (B). (C) Sirt2 mRNA expression levels in a panel of cells (extracted from http://biogps.org). (D,E) Sirt2 mRNA (D) and Sirtuin 2 (SIRT2) protein (E) expression in SIRT2+/+ and SIRT2−/− BMDMs assessed by RT-PCR and western blotting, respectively. Data are means ± SD from one experiment performed with three mice (D). (F) Sirt1-7 mRNA expression levels in SIRT2−/− BMDMs, expressed relative to the mRNA levels in SIRT2+/+ BMDMs set at 100%. Data are means ± SD from one experiment performed with three mice. A.U., arbitrary units. Full-length blots of panel (E) are presented in Figure S1 in Supplementary Material.