Table 1.
ELISAs of chemokine production by Raw 264.7 cells in response to CB-MPs.
4 h | 24 h | |||
---|---|---|---|---|
Cytokine | Control medium | CB-MPs | Control medium | CB-MPs |
MCP-1 | 69 ± 7 | 110 ± 53 | 220 ± 22 | 4, 040 ± 210 |
77 ± 43 | 1, 650 ± 130 | |||
58 ± 13 | 920 ± 30 | |||
MIP-1α | ND | ND | 10, 920 ± 430 | 17, 870 ± 330 |
10, 560 ± 210 | ||||
11, 940 ± 120 | ||||
TNF-α | 46 ± 6 | 1, 440 ± 100 | 59 ± 5 | 9, 880 ± 380 |
1, 570 ± 120 | 10, 150 ± 160 | |||
1, 980 ± 60 | 8, 800 ± 530 | |||
KC | ND | ND | <16 | 76 ± 20 |
Below LL | 76 ± 29 | |||
26 ± 10 |
ELISAs were performed using supernatants of cells exposed to CB-MPs or hydrolyzed CB dye. The concentrations are in pg/ml ± CI (α = 0.05). The three values per cell in the CB MP columns represent MCP-1, MIP-1α, and TNF-α responses to 10, 5, and 2.5% MP bed volumes, notated from top to bottom. For KC, the three values represent responses to 10, 3.3, and 1% bead volumes. ND, analysis not done. LL, concentration below the lower limit of quantification.