Figure 5.

Over expressions of nkx6.1 and olig2 rescued the motor neuron defects in insm1a deficient embryos. (A,B). Effects of insm1a knockdown on the expressions of nkx6.1 and olig2 at 19, 24, 36, and 48 hpf. Asterisks indicate significant differences (^*P < 0.05). (C) Abnormal Caps in insm1a knockdown zebrafish embryos were restored by co-injection of nkx6.1 or olig2 mRNA. Diagrams of Caps in dash line are displayed near the corresponding confocal image. Scale bar = 50 μm. (D) Quantification of zebrafish embryos with abnormal Cap primary motor neuron. Asterisks indicate significant differences (^**P < 0.01).