Fig. 4.

The Bg_9562 protein prevents mycelial growth and induces cell death responses in R. solani. a Effect of protein and buffer treatment on the growth of R. solani mycelia on agar slides. b Growth of buffer and 15 µg/ml Bg_9562 protein-treated R. solani mycelia on agar slides, measured in terms of observed growth area (cm²). c–f Microscopic view of buffer c and 15 µg/ml protein d–f treated mycelia. Leakage of cytoplasmic components from hyphae d, e along with deflated mycelia f were some of the prominent effects of protein treatment. The arrows highlight mycelial damage and asterisks indicate statistical significant difference between indicated groups at P < 0.001 (estimated by one-way ANOVA). Graphs show mean values ± s.d. The experiments were repeated at least three times with minimum three technical replicates and similar results were obtained