FIGURE 8.

The ros1-3 mutant shows hypermethylation of TE sequences in defense-related gene promoters, similar to rdd. DNA methylation in single demethylase mutants ros1-3, dml2-1, dml3-2 as well as the triple mutant rdd and WT was analyzed at 3 dpi. Methylation level was determined using Mutant Surveyor and the average of two biological replicates is shown as a heatmap. Each square represents a single cytosine in the sequenced region. Cytosines with significant hypermethylation (p ≤ 0.1) in ros1-3 (and rdd) compared to WT are indicated by red boxes. Regions where rdd and ros1-3 show significant differential methylation (p ≤ 0.1) are highlighted by blue boxes. Shoot data were largely similar and are not shown for easier visualization. Extended regions of non-differential methylation have been removed for easier visualization. The full dataset including the t-test p-values can be found in Extended Data File 2.