Table 1.
Details of the tested vaccine mixtures
| Animal model | Group | PTAI compositions or Lipofectin | Molar ratio PTAI: helper lipids | DNA dose | Ratioa |
|---|---|---|---|---|---|
| Chicken-broilers | 1 | PTAI-6-8 with DOPE | 1.5:1 | 125 μg | 0.9:1 |
| 2 | PTAI-11 with DOPE/DC | 1:1:1 | 125 μg | 0.8:1 | |
| 3 | PTAI-11 with DOPE/DC/DOPC | 1:1:1:1 | 125 μg | 1.3:1 | |
| 4 | PTAI-15 with DOPE/DC/DOPC | 1:1:1:1 | 125 μg | 1.6:1 | |
| 5 | Lipofectin | n.a | 125 μg | 1:6 | |
| 6 | PTAI-6-8 with DOPE or Lipofectinb | 1.5:1 | 125 μg | 0.9:1 or 1:6 | |
| Chicken-layers | 1 | PTAI-11 with DOPE/DC | 1:1:1 | 125 μg | 0.8:1 |
| 2 | PTAI-11 with DOPE/DC | 1:1:1 | 62.5 μg | 1.6:1 | |
| 3 | PTAI-11 with DOPE/DC | 1:1:1 | 62.5 μg | 0.8:1 | |
| 4 | Lipofectin | n.a | 125 μg | 1:6 | |
| 5 | Lipofectin | n.a | 62.5 μg | 1:6 | |
| 6 | None | n.a | 125 μg | n.a | |
| 7 | None | n.a | 62.5 μg | n.a | |
| 8 | Lipofectin | n.a | 125 μg | 1:6 | |
| Mice | 1 | Lipofectin | n.a | 10 μg | 1:6 |
| 2 | PTAI-10-14 with DOPE/DC | 1:1:1 | 10 μg | 0.8:1 | |
| 3 | PTAI-10-14 with DOPE/DC | 1:1:1 | 10 μg | 0.8:1 |
Vaccine mixtures were prepared by mixing the K3 plasmid with PTAI compositions or Lipofectin. Groups 6 and 7 (chicken-layers) were immunized with vaccine containing the K3 plasmid in PBS, pH 7.4 without any carriers. aratio for PTAI:DNA (w:w) or for Lipofectin:DNA (v:w) in vaccine doses is indicated; bin control group empty pCI vector was used with PTAI-6-8 (n = 3) or Lipofectin (n = 2); n.a not applicable