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. 2017 Sep 2;10:42. doi: 10.1186/s13041-017-0320-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression of endogenous PV-ChR2-EYFP in the nRT and labeling of the retrograde dye fluorogold that was injected in the S1 region. a ChR2 (green fluorescence) and fluorogold (yellow fluorescence). TC denotes thalamocortical relay neurons. b Enlarged thalamic region that shows cells that were retrogradely labeled with fluorogold in the thalamic region. c Enlarged thalamic region, showing that nRT neuron fibers (green fluorescence) surrounded thalamocortical neurons (fluorogold retrogradely labeled neurons). d Immunostaining of PV in the nRT. e Enlarged nRT region. Endogenous ChR2 was expressed only in the nRT. f Chemical fluorescence stain results of DAPI in the nRT. g Merged image of DAPI, PV, and ChR2