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. 2017 May 31;20(9):758–768. doi: 10.1093/ijnp/pyx041

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Published by Oxford University Press on behalf of CINP 2017. This work is written by (a) US Government employee(s) and is in the public domain in the US.

This Open Access article contains public sector information licensed under the Open Government Licence v2.0 (http://www.nationalarchives.gov.uk/doc/open-government-licence/version/2/).

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Figure 3. — Effects of adolescent alcohol exposure on the protein levels of α-melanocyte stimulating hormone (α-MSH) and melanocortin 4 receptor (MC4R) in the amygdaloid structures of rats in adulthood. (A) Representative photomicrographs (scale bar = 50 μm) of α-MSH staining in the central nucleus of the amygdala (CeA) and medial nucleus of the amygdala (MeA) of adolescent intermittent ethanol (AIE)- and saline (AIS)-exposed adult rats. (B) Bar diagram showing protein levels of α-MSH in the CeA, MeA, and basolateral amygdala (BLA) in AIE- and AIS-exposed adult rats as measured by gold immunolabeling. (C) Representative photomicrographs (scale bar = 50 μm) of MC4R gold immunolabeling in the CeA and MeA in AIE- and AIS-exposed adult rats. (D) Bar diagram showing protein levels of MC4R in the CeA, MeA, and BLA of AIS and AIE adult rats as measured by gold immunolabeling. Values are represented as the mean (±SEM) of the number of immunogold particles/100 μm² area. Values are significantly different from AIS-exposed adult group (**P<.01, *** P<.001, Student’s t test, n = 6).