Figure 6.
(A) Schematic representation showing location of forward and reverse primers in the NPY gene promoter used for the determination of histone acetylation overlapping with the DNA sequence that contains stretches of CpG islands. (B) Changes in histone H3K9/14 acetylation levels at the Npy gene promoter in the amygdala of AIS and AIE adult rats as analyzed by the chromatin immunoprecipitation (ChIP) assay. Values are represented as mean (±SEM) of fold change derived from AIS control group (**P<.01 significantly different from control AIS adult group, Student’s t test, n = 6–7). (C) Quantification of gold immunolabeling of NPY protein in the central (CeA), medial (MeA), and basolateral (BLA) nuclei of amygdala of AIS and AIE adult rats. Values are represented as the mean (±SEM) of the number of immunogold particles/100 μm2 area (***P<.001 significantly different from AIS control group, Student’s t test, n = 6). (D) Representative low-magnification photomicrographs (scale bar = 50 μm) of gold immunolabeling showing NPY-positive cells in the CeA and MeA of AIS and AIE adult rats.