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. 2017 Aug 10;108(9):1811–1819. doi: 10.1111/cas.13315

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© 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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MiR‐372 plays a negative role in cell proliferation, migration and invasion in human HPAC cells. BXPC‐3 and PANC‐1 cells were transfected with miR‐372 mimics or anti‐miR‐372 and used in following assay. (a) The expression levels of miR‐327 were detected by qRT‐PCR. (b) Cell proliferation rates were determined by the CCK8 assay. *P < 0.01 versus negative control (NC). (c) Cell apoptosis was determined by flow cytometry. (d) Cell proliferation and apoptosis were detected by EdU (Scale bar: 100 μm) and Hoechst 33 258 (400 × magnification) staining assay. (e,f) Migration and invasion were determined by transwell migration (200 × magnification) assay, transwell invasion (200 × magnification) assay and wound scratch assay (40 × magnification).