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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Clin Cancer Res. 2017 May 24;23(17):5202–5209. doi: 10.1158/1078-0432.CCR-16-3107

Figure 4.

Figure 4

Detection of B7-H3 and B7-H4 protein expression to assess co-localization using immunofluorescence (QIF) in lung cancer. A) Representative fluorescence images showing the simultaneous detection of B7-H3 (red channel), B7-H4 (blue channel) and cytokeratin (green channel, inserted box is highlighting the area that has been magnified in figure 2 C–F. B) Simultaneous detection of B7-H3 (red channel), B7-H4 (blue channel) C) 4′,6-diamidino-2-phenylindole (DAPI) only in purple channel, D) Cytokeratin only in green channel, E) B7-H3 only in red channel, D) B7-H4 only in blue channel. Bar=100 μm.