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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Clin Cancer Res. 2017 Jun 9;23(17):5281–5291. doi: 10.1158/1078-0432.CCR-17-0171

Figure 3.

Figure 3

miR-193a does not have increased DNA methylation in UC-associated neoplasia. A) Genomic structure of miR193a-3p. Location of miR-193a (gray rectangle) with respect to CpG sites (vertical lines) and region examined by bisulfite genomic sequencing. The analyzed genomic region had 27 CpG dinucleotides as shown in red. The genomic sequence of pre-miR-193a is underlined. B) Bisulfite genomic sequencing in eight subjects, including three normal controls, two UC without dysplasia, and three UC-associated cancers. Red boxes represent methylated CpG sites and blue boxes unmethylated sites. C) Real time PCR of pri-miR-193a and miR-193a-3p following treatment with 0, 5μM, or 10 μM of 5-aza-2’-deoxycytidine daily for 5 days in the indicated colon cancer cell lines (*p <0.05).