Figure 4.

miR-193a-3p is down-regulated and IL17RD up-regulated in UC-associated cancer A) Expression of the primary transcript of miR-193a and mature miR-193a-3p in an independent validation cohort of UC-associated cancers and adjacent tissue as compared to normal controls (n=11/group) B) Expression of miR-193a-3p, in whole colon biopsies, the fractionated colonocytes, and stroma from the same normal control patients (n=13). Vimentin, which is highly expressed in colonic fibroblasts, and CK20 which is expressed in colonocytes but not stromal cells, were measured as cell purity controls. C) Expression of IL17RD in UC-associated cancers and adjacent tissue as compared to normal controls (n=7/group). D) Correlation between miR-193a-3p and IL17RD transcripts. Individual sample expression of miR-193a-3p as compared to expression IL17RD with logarithmic trend line in IBD-cancers and adjacent tissue (fold change of expression as compared to normal controls). Samples included had expression data available for both miR-193a-3p and IL17RD (n=12, UC-cancer=6, adjacent tissue=6). E) Immunohistochemistry of IL17RD at 10× magnification [a: Normal Control; b: Quiescent UC; c: Active UC; d,e: Non-dysplastic mucosa adjacent to a cancer; f,g: UC-cancer; h,i: Sporadic colon cancer; j,k,l: Representative images from UC-cancers in tissue array demonstrating positive staining in epithelial cancer cells