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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Neurobiol Aging. 2017 Jun 24;58:88–101. doi: 10.1016/j.neurobiolaging.2017.06.014

Table 1.

PCR Primer Description and Sequences

Gene Primer Sequence: 5′ -> 3′ Function
β-Actin F: TTCCTTCCTGGGTATGGAAT
R: GAGGAGCAATGATCTTGATC		 Cytoskeletal protein (housekeeping gene)
CD11b F: CTGGGAGATGTGAATGGAG
R: ACTGATGCTGGCTACTGATG		 Macrophage/Microglial antigen marker
Iba-1 F: GGCAATGGAGATATCGATAT
R: AGAATCATTCTCAAGATGGC		 Macrophage/Microglial antigen marker
MHCII F: AGAFACCATCTGGAGACTTG
R: CATCTGGGGTGTTGTTGGA		 Macrophage/Microglial antigen marker
NLRP3 F: AGAAGCTGGGGTTGGTGAATT
R: GTTGTCTAACTCCAGCATCTG		 Rate limiting protein in NLRP3 inflammasome formation
HMGB1 F: GAGGTGGAAGACCATGTCTG
R: AAGAAGAAGGCCGAAGGAGG		 Endogenous danger signal

Abbreviations: CD11b: cluster of differentiation 11b; Iba-1: ionized calcium-binding adaptor molecule-1; MHCII: Major Histocompatibility Complex II; NLRP3: nod-like receptor protein 3; HMGB1: high mobility group box 1