(a, b) Co-immunofluorescence for NFATc1/Ki67, K15/Ki67 and Sox9/Ki67 in control and DTG HF under conditions of 36 hours post-depilation at P21 (a), and pre-depilation at P21 (b), Pretreated with Dox at P18; (c, d) Co-immunofluorescence for NFATc1/Ki67, K15/Ki67 and Sox9/Ki67 in control and cKO HFs 36 hours post-depilation at P21 (c) and pre-depilation at P21 (d). The dashed line: boundary of BU and HG. Arrows: the double positive signal. (a-d) Quantification of NFATc1+Ki67+, K15+/Ki67+ and Sox9+Ki67+ cells in the corresponding conditions. n = 3. * p<0.05; ** p<0.01;*** p<0.001. Scale bar, 50 mm.